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3
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[Original]
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Occurrence and Molecular Analysis of Malassezia spp. in the External Ear Canal of Dogs
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Takashi CHIBA, Kaoru HATAKEYAMA, Hiroshi MIZUTANI,
Tomoaki WAUKE, Yumi TAKAHASHI,
Sumio YAMADA, Akemi KAI and Kazuyoshi YANO
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Malassezia yeasts are normal skin flora of human and animals. However, hospitals have acquired infections caused by Malassezia spp. have been reported recently. The aim of this study was to evaluate the occurrence and DNA sequence diversity of Malassezia spp. in the external ear canal of dogs in the Tokyo Metropolitan Area.
As a result Malassezia spp. were isolated from 33 out of 52 (63.5%) dogs. M. pachydermaitis was identified in 31 (59.6%) specimens by rDNA sequence analysis. These 31 isolates were classified into 5 clusters by molecular phylogenetic analysis using ITS1 region. Three isolates from 3 out of 52 specimens were found to display a high rDNA region sequence similarity to that of M. japonica type strain.
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Yeast, Malassezia spp., Dog, Molecular Phylogenetic Tree
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
49-52,
2007 |
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4
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[Original]
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Clostridium perfringens with Delayed Lactose Fermentation Isolated from a Foodborne Outbreak
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Chie MONMA, Hiromi OBATA, Noriko KONISHI, Kaoru HATAKEYAMA, Yoko IKEUCHI, Akemi KAI, Yoshitoki YANAGAWA and Kazuyoshi YANO
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A foodborne outbreak of gastroenteritis occurred among persons who had eaten Chinese food in Tokyo between 20 and 27 December, 2000. As a result of epidemiological investigation, a total of 101 patients (18 groups) of 685 persons (42 groups) who had eaten causal food was reported. Clostridium perfringens serotype TW47, which produces enterotoxin was isolated from 12 of 50 patients, 1 of 12 food handlers and 2 of 8 foods at the restaurant and regarded as the cause of the foodborne outbreak. The isolate was atypical strain that the color of lecithinase reaction on CW agar with egg yolk was orange color, atypical color. We compared pH of broth cultures after inoculated the isolates from the outbreak and that of isolates from the others. After 24 hours incubation, pH of cultures after incubated the isolates from the outbreak showed pH 5.0-6.1(pH 5.4 on average) , though pH of cultures after incubated the isolates from the other outbreaks showed pH 4.5-5.1 (pH 4.8 on average). It seemed that the lecithinase reaction of the isolate colored orange because the lactose fermentation of the isolate was delayed than that of the other outbreaks' isolates. The isolate from the outbreak had typical characteristics of C. perfringens except lecithinase reaction, and 16S rDNA of C. perfringens.
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Clostridium perfringens, foodborne outbreak, lecithinase reaction, delayed lactose fermentation, atypical characteristics
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
53-56,
2007 |
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5
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[Original]
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Usefulness of Variable Numbers of Tandem Repeats Method as a Molecular Epidemiological Tool for Outbreak of Mycobacterium tuberculosis
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Jun MUKAIGAWA, Hirofumi MIYAKE, Yoshitoki YANAGAWA and Kazuyoshi YANO
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outbreak of Mycobacterium tuberculosis, IS6110-restriction fragment length polymorphism method, variable numbers of tandem repeats method
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
57-61,
2007 |
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6
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[Original]
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Detection of Human Papillomavirus from the Specimens of Sentinels for Infectious Agent Surveillance
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Mami NAGASHIMA, Kenji SADAMASU, Takayuki SHINKAI, Kazue OGATA, Yasuko YOSHIDA and Kazuyoshi YANO
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human papillomavirus, multiplex PCR, sequencing analysis, low risk HPV, high risk HPV
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
63-66,
2007 |
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7
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[Original]
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Detection of Influenza Virus in Sentinels for Infectious Agent Surveillance in Tokyo (2006 / 2007 season)
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Takayuki SHINKAI, Kenji SADAMASU, Mami NAGASHIMA, Kazue OGATA, Yasuko YOSHIDA, Kazuyoshi YANO, Satoshi AMAKI, Kazuko KASHIWADA, Takashi KATO, Junichi KATAHIRA, Takashi KAMO, Isafumi KIDOKORO, Masahide SHIOZAKI, Masakazu SUZUKI, Mitsuhiko TANAKA, Shunkichi HIRATA, Minoru HOUJHO, Teiichi MATSUNAGA, Keiichi YAMAGAMI and Norio YAMAGUCHI
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sentinel for infectious agent surveillance, influenza virus, sequencing analysis, virus isolation test
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
67-72,
2007 |
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8
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[Information]
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Attempt of Detection of Drug Resistant Bacteria from Canine Stools
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Kaoru HATAKEYAMA, Rumi OKUNO, Miyoko ENDO and Yoshitoki YANAGAWA
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drug resistant bacteria, methicillin-resistant Staphyrococcus aureus, vancomycin resistant enterococci, extended-spectrum β lactamase
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
73-76,
2007 |
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9
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[Information]
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Evaluation of Utility of Reagent for Anti-syphilis Antibodies Using a Latex Agglutination on an Automatic Analyzer
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Hiroki TAKANO, Iku ISE and Yoshitoki YANAGAWA
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syphilis, serologic test for syphilis method, TP method, latex agglutination
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| Ann.Rep.Tokyo Metr.Inst.P.H., 58,
77-80,
2007 |