56 Simple Analysis of the Brominated Flame-retardant, Tetrabromobisphenol A, in Mice Tissues
|
| Tomoko FUJITANI, Yukie TADA, Hiroshi TAKAHASHI, Norio YANO, Katsuhiro YUZAWA, Akemichi NAGASAWA, Hiroshi ANDOH, Yoshikazu KUBO, Akio OGATA and Hisashi KAMIMURA |
| Tetrabromobisphenol A (TBBPA), a brominated flame-retardant, has been widely detected in the environment, wildlife, and human plasma, and is now under concideration for adverse effects in experimental animals by embryonal, fetal, and neonatal exposure in our laboratory. In order to determine the maternal-fetal distribution of TBBPA, simple methods for sample preparation from tissues or body fluids, and HPLC analysis were investigated. Using a reverse-phase analytical column, a two isocratic step system with mobile phase A (80% methanol, 1 mM ammonium acetate) from 0 to 15 min for separation of TBBPA and mobile phase B (100% methanol) from 15 to 22 min for column washing, a flow rate of 0.8 ml/min, and detection at 295 nm, TBBPA concentrations could be quantitatively determined with a lower limit of 1.0 ng. Supernatant from homogenates of tissues in methanol were suitable for HPLC analysis, with a detection limit of 20 ng/g tissue wet weight. For the detection of TBBPA in blood, diethyl ether extraction was suitable. |
| brominated flame-retardant, tetrabromobisphenol A, HPLC analysis, distribution, placental transfer, milk excretion, mice |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,327-331,2005 |
|
| 57 Hormonal Effects of Organic Phosphate Triesters Study by the Reporter Gene Assays
|
| Ken-ichi OHYAMA, Saeko NAGATA, Naoko HOSOGOE, Aya ONUKI, Ikue SAITO and Hiroshi SETO |
| Organic phosphate triesters used as a flame retardant have been identified in indoor air, and it is apprehended that these compounds may affect human health. Therefore, we examined the androgenic and the estrogenic activities of organic phosphate triesters (11 compounds) and carried out a luciferase reporter-gene assay using cultured cells. The organic phosphate triesters examined were trimethylphosphate (TMP), triethylphosphate (TEP), tripropylphosphate (TPP), tributylphosphate (TBP), tris(2-chloroisopropyl)phosphate (TCIPP), tris(2-chloroethyl)phosphate (TCEP), tris(2-ethylhexyl)phosphate (TEHP), tri(butoxyethyl)phosphate (TBEP), tris(1,3-dichloro-2-propyl)phosphate (TDCPP), triphenylphosphate (TPHP), and tricresylphosphate (TCP). TPP, TBP, TCIPP, TCEP, TBEP, TDCPP, TPHP and TCP inhibited the luciferase expression induced by dihydrotestosterone (10-9 M) and the IC50 value was 4.7 x 10-5 - 6.0 x 10-4 M. Moreover, TBP, TCIPP, TBEP, TDCPP, TPHP, and TCP inhibited the luciferase expression induced by 17β-estradiol (3 x 10-10 M) and the IC50 value was 3.3 x 10-5 - 2.3 x 10-4 M. It is suggested that some organic triesters have antiandrogenic and/or antiestrogenic activities. |
| organic phosphate triesters, antiandrogenicity, antiestrogenicity, reporter-gene assay |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,333-338,2005 |
|
| 58 Effect of Natural Food Additives on Proliferation of Human Brest Cancer MCF-7 Cell
|
| Hiroshi FUJITA, Akio OGATA and Hisashi KAMIMURA |
| natural food additives, endocrine disrupter, estrogen, lac color, MCF-7 |
| Ann.Rep.Tokyo Metr.Inst.P.H.,
56,339-341,2005 |
|
| 59 Uetrotrophic Effect of 2,4-di-tert-Butylphenol on Ovariectomized CD1 Mice
|
| Yoshimitsu SAKAMOTO, Kanako SATOH, Hiroshi ANDO, Yoshikazu KUBO, Akemichi NAGASAWA, Norio YANO, Katsuhiro YUZAWA, Hiroshi TAKAHASHI, Akio OGATA and Hisashi KAMIMURA |
| 2,4-di-tert-Butylphenol (BP) is one of the migrants from disposable glove made from nitril-butadiene rubber and seems to be the degradation product of antioxidant used for nitril-butadiene rubber. In this study, uterotrophic effect of BP was observed in ovariectomized (OVX) CD1 mice. OVX mice were given BP by oral gavage at dose of 10, 50, 250 mg/kgBW for 3 days. Body weight and uterine weight of BP treated groups were not different from those of control group. Histologically, obvious changes in uteri and vagina were not observed in BP treated group compared with control group. In the DES group, uterine weight increased 400% compared to OVX control group and marked increase in luminal epithelial height and stratification with cornification of vaginal epithelium were observed. In this study, uterotrophic effect of BP was not observed in the range of 10 to 250 mg/kg by oral gavage. |
| disposable glove, nitrile-butadien rubber, migrants, 2,4-di-tert-butylphenol, uterotrophic study, ovariectomoized mice |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,343-346,2005 |
|
| 60 2-Hydroxy-4-methoxybenzophenone, an UV-Absorber, Induces Xenoestrogenic Effects through Some Intermediary Metabolites
|
| Yoshio NAKAGAWA, Fumiko NAGAI and Toshinari SUZUKI |
| 2-hydroxy-4-methoxybenzophenone, UV absorber, metabolism, estrogenic effect, cytotoxicity, hepatocytes, MCF-7 cells |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,347-352,2005 |
|
| 61 Examination of Biological Effects on Mice Administrated Sodium Picosulfate Supposed Containing in Dietary Supplements
|
| Nobutaka FUKUMORI, Hiroshi ANDO, Yoshikazu KUBO, Katsuhiro YUZAWA, Akemichi NAGASAWA, Hiroshi TAKAHASHI, Norio YANO and Akio OGATA, Hisashi KAMIMURA |
| sodium picosulfate, dietary supplement, biological effects, mouse |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,353-357,2005 |
|
| 62 Oral Toxicity Test of N-Nitrosofenfluramine in Chinese Weight-loss Dietary Supplement –Pathological Study–
|
| Yukie TADA, Kanako SATOH, Ryouichi NONAKA, Arisa YAMADA, Tsuyoshi SATOH, Nobutaka FUKUMORI, Tomoko FUJITANI, Norio YANO, Hiroshi TAKAHASHI, Katsuhiro YUZAWA, Hiroshi ANDOH, Yoshikazu KUBO, Akemichi NAGASAWA, Fumiko NAGAI, Akio OGATA and Hisashi KAMIMURA |
| chinese weight-loss dietary supplements, N-nitrosofenfluramine, mouse, oral administration, pathological study |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,359-362,2005 |
|
63 Chromosomal Aberration Test of Para Tea in Chinese Hamster
|
| Seiji YOSHIDA, Akio OGATA and HIisashi KAMIMURA |
| para tea, chromosome analysis, chinese hamster |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,363-366,2005 |