6 Detection of Medicines from Non-permitted Drugs Being on the Market for the Purpose of Atopic Dermatitis Pharmacotherapy
|
| Keiko MINOWA, Kiyoko KISHIMOTO, Takako MORIYASU, Sutemi SHIGEOKA and Ichiro YASUDA |
| non-permitted drugs, clobetasol propionate, ketoconazole, HPLC/PDA, pattern analysis, optical isomer, optical rotation, atopic dermatitis |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,47-51,2005 |
|
| 7 The Stability of Hydroquinone in the Anti-pigmentation Compound
|
| Kiyoko KISHIMOTO, Keiko MINOWA, Takako MORIYASU, Sutemi SHIGEOKA and Ichiro YASUDA |
| anti-pigmentation compound, ointment, hydroquinone, stability, long term testing, accelerated testing, HPLC, infrared spectra |
| Ann.Rep.Tokyo Metr.Inst.P.H.,
56,53-58,2005 |
|
| 8 Analytical Methods and Spectrum Data of 4 Governor-designated Drugs
|
| Machiko NAGASHIMA, Takako SETO, Misako TAKAHASHI, Jin SUZUKI and Ichiro YASUDA |
| 2,5-dimethoxy-4-iodophenethylamine, N-methyl-α-ethyl-3,4-methylenedioxyphenethyl amine, 3-[2-(isopropylmethylamino)ethyl]-5-methoxyindole, 1-(3-chlorophenyl)- piperazine, Governor-designated Drug |
| Ann.Rep.Tokyo Metr.Inst.P.H.,
56,59-64,2005 |
|
| 9 Analysis of Anesthetics in Uncontrolled Drugs
|
| Misako TAKAHASHI, Jin SUZUKI, Machiko NAGASHIMA, Takako SETO and Ichirou YASUDA |
| Anesthetics and their analogues are often used in uncontrolled drugs. We tried to analyze seven components of downer drugs simultaneously. The seven drug components were; 4-hydroxybutyric acid (GHB), 4-butyrolactone (GBL), 1,4-butanediol, 4-valerolactone, methylphenidate hydrochloride, ketamine hydrochloride, and dextromethorphan hydrobromide, and there were investigated by GC and GC/MS. The compounds were satisfactorily separated by GC; however, GHB converts to GBL in the heated injection ports of the GC conditions. Therefore, GHB was analyzed using HPLC. Each compound could be identified its by mass spectra. We established the analysis method of downer drugs by GC, GC/MS and HPLC. This analysis method was able to be applied to uncontrolled drug on the market. |
| anesthetics, uncontrolled drugs, downer drugs, GC, GC/MS, HPLC |
| Ann.Rep.Tokyo Metr.Inst.P.H.,
56,65-68,2005 |
|
| 10 Measurement of Purity of Governor-Designated Drugs and Estimated Structural Formula of the Impurities
|
| Jin SUZUKI, Takako SETO, Machiko NAGASHIMA, Misako TAKAHASHI, Chiyomi OKUMOTO and Ichiro YASUDA |
| The purity of governor-designated drugs (2,5-dimethoxy-4-iodophenethylamine (2C-I), N-methyl-α-ethyl-3,4-methylenedioxyphenethylamine (MBDB), 3-[2-(isopropylmethylamino)ethyl]-5-methoxyindole (5-MeO-MIPT), and 3-chlorophenylpiperazine (3CPP)) used for the purpose of living body influence evaluation was measured. The samples were analysed by using TLC, GC-FID, IC, HPLC-PDA, and NMR. Using TLC and GC-FID methods, only a main spot and a single peak was detected in all samples. Using IC, the form of exsitence of all compounds was clarified, and using HPLC-PDA and NMR methods, the purity of all samples was calculated with to be with over 90%. |
| a governor-designated drug, purity, impurity, TLC, IC, GC, HPLC, NMR |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,69-74,2005 |
|
| 11 The Identifications and the Aspects of the Commercially Available Uncontrolled Drugs Purchased between Apr. 2003 and Mar. 2004
|
| Takako SETO, Misako TAKAHASHI, Matiko NAGASHIMA, Jin SUZUKI and Ichiro YASUDA |
| In total 31 kinds of chemical drugs were found in 100 samples of commercially available uncontrolled drugs which were purchased between Apr. 2003 and Mar. 2004 in Tokyo. Of those, 21 drugs were detected for the first time by us. Their respective structures were identified by a combination of 1H-NMR, 13C-NMR, LC/PDA, LC/MS (ESI), HR/LC/MS (ESI), GC/MS (EI) and TLC. |
| uncontrolled drug, chemical drug, NMR, LC/MS, GC/MS, TLC, LC/PDA, identification |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,75-80,2005 |
|
| 12 Determination of Drugs in the Dietary Supplements for Weight Loss and Appling to "Tentenso" Caused Health Damage
|
| Takako MORIYASU, Keiko MINOWA, Kiyoko KISHIMOTO, Sutemi SHIGEOKA, Hideo KADOI and Ichiro YASUDA |
| dietary supplements, weight loss, suppression appetite, advance metabolism, purgative, diuretic, TLC, HPLC/PDA, weight loss capsule "tentenso" |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,81-86,2005 |
|
| 13 Contents of Hypericin and Hyperforin in the Health Foods Mixed with St. John's Wort
|
| Tomoko HAMANO, Hiroko SHIODA, Junichi NAKAJIMA and Ichiro YASUDA |
| An HPLC method for the
determination of the concentration of hypericin and hyperforin in St. John’s
Wort (SJW), the components with antidepressive effects, was established.
From the quantitative analysis of 40 kinds of the health foods mixed with
SJW, it was shown that the concentration of both components varied widely,
particularly hyperforin. In more than 80% of the products the maximum intake
per day, calculated from the quantitative values, as equal to or over the
concentration of the drug as used in Europe. The different intensities of
the antidepressive effects and the potential for drug interactions caused by
the dispersion of SJW in health foods may possibly to lead to health risks. |
| St. John's Wort, hypericin, hyperforin, contents, intake, HPLC, health food, antidepressive effect, drug interaction |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,87-91,2005 |
|
| 14 Rapid Pretreatment Using Solid Phase Extraction for Analyzing Pesticide Residues in Crude Drugs
|
| Junichi NAKAJIMA, Tomoko HAMANO, Hiroko SHIODA and Ichiro YASUDA |
| To rapidly prepare test solutions for the analysis of pesticides α-BHC, β-BHC, γ-BHC, δ-BHC, o,p'-DDT, p,p'-DDT, p,p'-DDD, p,p'-DDE, cypermethrin, fenvalerate, and cis and trans permethrin in crude drugs, the use of ready-to-use cartridges filled with a macroporous diatomaceous material was investigated. This method (A) was compared with a Japanese Pharmacopea extraction method (B) using a liquid - liquid partitioning procedure. N-haxane was the most suitable solvent, and 200 mL was sufficient to elute target pesticides from the cartridges. It was found that acetone in the aqueous sample loading solution, was the main cause of error. Allowing for this, there was no difference between methods A and B regarding recovery, contents of pesticides,or repeatability. Compared with method B, method A was considered to be a reliable and useful extraction method because the protocol was simple, and the troublesome emulsions that were frequently observed in the liquid - liquid partitioning procedure did not occur. |
| rapid pretreatment, solid phase extraction, crude drugs, pesticide residue, organochlorine pesticicide, pyrethroid pesticide, GC-ECD, repeatability. |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,93-97,2005 |
|
| 15 Identification of Echinacea in Supplement Using Scanning Electron Microscope
|
| Masako ARAGANE, Tatuo FUKUDA, Masao YOSHIZAWA, Yukiko SUZUKI, Youji MORIMOTO, Tomoko HAMANO, Nobutaka FUKUMORI and Ichiro YASUDA |
| Echinacea, identification, scanning electron microscope (SEM), herb supplement |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,99-104,2005 |
|
| 16 Simultaneous Determination of UV Absorbents in Quasi-Drugs and Cosmetics
|
| Toshiro YOKOYAMA, Ken'ichiro MORI, Yoshiaki NAKAMURA, Kiyoshi TERAJIMA, Nahomi OHNUKI and Shuzo OGINO |
A method for the simultaneous determination of twelve UV absorbents in quasi-drugs and cosmetics was developed. The chemical name and abbreviations of the twelve UV absorbents are as follows, ethyl p-aminobenzoate (EAB), tetrahydroxybenzophenone (THB), ethoxyethyl p-methoxycinnamate (Cinoxate), dihydroxybenzophenone (DHB), dihydroxydimethoxy-benzophenone (DHDMB), 2-hydroxy-4-methoxybenzophenone (HMB), 2-ethylhexyl (Z)-4-(3,4-dimethoxybenzylidene)-2,5-dioxo-1-imidazolyzine propionate (EBP), 2-ethylhexyl p-dimethylaminobenzoate (EDB), 4-tert-butyl-4'-methoxydibenzoylmethane (BMB), 2-ethylhexyl p-methoxy-cinnamate (EMC), 2-ethylhexyl salicylate (ES) and 2,2'-methylene-bis-[6-(2H-benzotriazole-2-yl) -4-(1,1,3,3-tetramethylbutyl)- phenol] (MBP).
A quasi-drugs or cosmetics sample of 1 g was weighed accurately, mixed with tetrahydrofurane (THF), dispersed by treatment in a supersonic water bath, and made up to 50 mL with THF. The solution was filtered through a 0.45 µm membrane filter, and injected into on HPLC. Analytical conditions were as follows, column: TSK-GEL ODS-80TS (4.6 mm i.d. × 250 mm), column temperature:40°C, mobil phase: gradient, THF: water (40 : 60) to (80 : 20), 1 ml/min, injection volume : 10 µL, detector: UV 310 nm. By this method, the concentration of twelve UV absorbents in ten commercial products were determined with high precision and no cross-interference. |
| UV absorbents, quasi-drugs, cosmetics, simultaneous determination |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,105-110,2005 |
|
| 17 Simultaneous Determination of Photosensitizers in Quasi-Drugs and Cosmetics
|
| Ken'ichiro MORI, Yosiaki NAKAMURA, Nahomi OHNUKI, Kiyoshi TERASHIMA, Toshiro YOKOYAMA and Shuzo OGINO |
| A method for the simultaneous determination of five photosensitizers, PS101, PS201, PS301, PS401 and Plalumin in quasi-drugs and cosmetics was developed. A 1.0 g of sample was dissolved with methanol, and dispersed for fifteen minutes by immersion in a supersonic water bath, and made up to 5 mL with methanol. The solution was filtered using a 0.45 µm membrane filter. This sample solution and the standard solution of photosensitizers were prepared just before analysis and stored in the dark to avoid photodegradation of the photosensitizers. These solutions 20 µL were injected into an HPLC. The analytical conditions were as follows, ODS column, stepwise gradient mobil phase; acetonitrile : 0.05 mol/L triethylamin, 0.1 mol/L phosphoric acid (25:75) to (80:20), photodiode array detector. There was no influence from typical co-existent, ingredients such as antiseptics or UV absorbents, in the quasi-drugs and cosmetics tested. Recovery of the five photosensitizers spiked into commercial cosmetics was 95.0 - 102.0%, C.V.(%) 0.7 - 3.8. |
| cosmetics, quasi-drugs, photosensitizers, photosensitizing dye 101, quaternium-73, quaternium-51, quaternium-45, plalumin, HPLC |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,111-115,2005 |
|
| 18 Analysis of New Hair Colorants for Cosmetics
|
| Nahomi OHNUKI, Yoshiaki NAKAMURA, Ken'ichiro MORI, Kiyoshi TERAJIMA, Toshiro YOKOYAMA and Shuzo OGINO |
| A rapid and simple analysis by HPLC was developed to determine the 20 synthetic hair colorants in cosmetics. The analytical procedure was as follows: the samples were diluted with 50% methanol and injected into the HPLC. The HPLC conditions were as follows: HPLC column; ODS (4.6 mm i.d.×150 mm), mobile phase. 0.01 mol/L phosphate buffer including 0.5% sodium 1-decanesulfonate, pH 2.5/Acetonitrile, stepwise condition, photodiodearray; 200 - 800 nm, column temperature; 40°C. Twenty hair colorants were separated for about 40 min and were identified from the absorbence spectra of each peak. By using this method, hair colorants used in 10 commercial cosmetics were identified, quantified, and shown to have good handling qualies. |
| HPLC, cosmetics, hair colorant, semipermanent hair dye preparation, analysis |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,117-121,2005 |
|
| 19 Determination of Zinc Oxide in Talcum Powder by X-Ray Fluorescence Spectrometry
|
| Yoshiaki NAKAMURA, Ken'ichiro Mori, Kiyoshi TERAJIMA, Nahomi OHNUKI, Toshiro YOKOYAMA and Syuzo OGINO |
| An analytical method for the detection of Zinc Oxide (ZnO) by X-ray fluorescence spectrometry (XRFS) in talcum powder was developed. A talcum powder sample (1.0g) and 2.0 g of cellulose powder were mixed, pressed into a briquette, and analyzed by XRFS. XRFS conditions were as follows: Target: Rh/Cr, Voltage (kv): 50, Current (mA): 50, Crystal: LiF (200), Detector: Scintillation Counter, Spectrum: Zn-kα 2θ. The recovery of ZnO in spiked talcum powders as 97.9 - 100.9%. The detection limit was 0.005%. By this method, eleven commercial talcum powder samples were analyzed, and a ZnO level of 2.0 - 10.3% was detected in nine samples with a high accuracy and with no interference from other ingredients. |
| zinc oxide, talcum powder, X-Ray fluorescence spectrometry, quasi-drugs |
| Ann.Rep.Tokyo Metr.Inst.P.H., 56,123-126,2005 |