61 Immune Response to Cryptomeria japonica Pollen in Rats Exposed to Low Dose Diesel Exhaust During Fetal and Suckling Periods
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| Nobue WATANABE, Masanobu OHSAWA, Katsuhiro YUZAWA, Norio YANO, Akimichi NAGASAWA, Yoshikazu KUBO, Hiroshi TAKAHASHI, Hiroshi ANDO, Akio OGATA and Shin-ichi UEHARA |
| This study was conducted to determine the non-effect dose of diesel exhaust exposure on immune responses against pollen. Pregnant rats were divided into nine groups: Group 1, exposed to clean air (Control), Groups 2, 3, 4 and 5, exposed to total or filtered diesel exhaust during the fetal period (HT-C-C, LT-C-C, HF-C-C, LF-C-C), and Groups 6, 7, 8 and 9, exposed to total or filtered diesel exhaust during the suckling period (C-HT-C, C-LH-C, C-HF-C, C-LF-C). Total diesel exhaust contained 1.71 mg/m3 particulate matter and 0.80 ppm nitrogen dioxide (high dose) or 0.17 mg/m3 particulate matter and 0.10 ppm nitrogen dioxide (low dose). Filtered exhaust without particles contained 0.80 (high dose) or 0.10 (low dose) ppm nitrogen dioxide. Intraperitoneal injection of 5 mg crude cedar pollen was performed 3 or 4 times at 2-week intervals from the 49th day after birth. The IgE titers measured by P-K reaction of the LT-C-C and LF-C-C groups elevated to the same levels of those of the HT-C-C and HF-C-C groups and were significantly higher than those of the Control group after the 3rd immunization. The IgE titers in the C-LH-C and C-LF-C groups elevated to the same levels of those of the C-HT-C and C-HF-C groups and were significantly higher than those of the Control group after the 4th immunization. The threshold dose of diesel exhaust exposure that elevates IgE against pollen could be lower than the dose used in this study. |
| diesel exhaust, rat, Cryptomeria japonica pollen, IgE, fetal-period, suckling periods, interleukin, mast cell |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,325-331,2003 |
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| 62 Pathological Changes of Female Reproductive System Exposed to Diesel Exhaust during Fetal, Suckling and Weaning Period
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| Nobue WATANABE, Ayana NISHINAKA, Tomoka TAMADA and Masanobu OHSAWA |
| diesel exhaust, rat, ovary, follicle stimulating hormone, luteinizing hormone, fetal-period, suckling-period, weaning-period |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,332-336,2003 |
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| 63 Toxicity of Bisphenol A on Testis and Male Accessory Organs in Rats and Mice (I). Dose-Response Relationship and Species Differences
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| Osamu TAKAHASHI, Toyohito TANAKA, Shinshi OISHI, Fumiko NAGAI, Akemichi NAGASAWA, Katsuhiro YUZAWA, Hiroshi TAKAHASHI, Norio YANO, Yoshikazu KUBO, Hiroshi ANDO, Akio OGATA, Hisashi KAMINURA and Itsu KANO |
| bisphenol A, testis, male accessory organs, rat, mouse, toxicity, species differences, endocrine disruptors |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,337-341,2003 |
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| 64 Toxicity of Bisphenol A on Testis and Male Accessory Organs in Rats and Mice (II). Administration-Route Dependency
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| Osamu TAKAHASHI, Toyohito TANAKA, Shinshi OISHI, Fumiko NAGAI, Akemichi NAGASAWA, Katsuhiro YUZAWA, Hiroshi TAKAHASHI, Norio YANO, Yoshikazu KUBO, Hiroshi ANDO, Akio OGATA, Hisashi KAMINURA and Itsu KANO |
| bisphenol A, testis, male accessory organs, rat, mouse, toxicity, administration-route, endocrine disruptors |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,342-346,2003 |
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| 65 Low Dose Effects of Bisphenol A on the Ultrastructure of Prostate in Suckling Male Rats
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| Nobutaka FUKUMORI, Kuniaki TAYAMA, Hiroshi ANDO, Yoshikazu KUBO, Norio YANO, Hiroshi TAKAHASHI, Akemichi NAGASAWA, Katsuhiro YUZAWA, Yoshimitsu SAKAMOTO and Akio OGATA |
| Low dose effects of bisphenol A (BPA) on the ultrastructure of ventral prostate in suckling male rats were investigated using thin-section electron microscopy. BPA (0, 0.8, 4, 20, 500 µg/kg) or 17β-estradiol (E2:100 µg/kg) dissolved in DMSO were subcutaneously administered to suckling male rats from Day 1 through Day 21 after birth. An increase in secretory granules accompanied by (or coupled with) the disappearance of microvilli on the surface of the glandular epithelium and the proliferation of fibroblasts in the fibromuscular layer of the stroma were observed in the E2-treated rats. The secretory epithelial cell height of the prostate in the rats treated with E2 was essentially unchanged in comparison with those of the rats treated with DMSO only. Though a slight increase in secretory granules in the glandular epithelium was observed in the rats treated with 20 and 500 µg BPA/kg, the alteration in the stroma was not salient in comparison with that of the rats treated with E2. These findings suggest that a low dose of BPA may exert estrogenic effects on the ventral prostate in suckling male rats at the ultrastructural level. |
| bisphenol A, 17β-estradiol, prostate gland, rat, electron microscopic observation |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,347-352,2003 |
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| 66 Modulation of Mutagenic Activity of the Known Mutagens by Food Additives in the Ames Test (IV)
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| Hiroshi FUJITA and Akio OGATA |
| antimutagenicity, co-mutagenicity, food additives, Ames test |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,353-356,2003 |
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| 67 Cytogenetic Studies of Genetically Modified Soybean (II)
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| Seiji YOSHIDA, Yoshimitsu Sakamoto, Yukie TADA, Norio YANO, Katsuhiro YUZAWA, Akemichi NAGASAWA, Hiroshi TAKAHASHI, Hiroshi ANDO, Yoshikazu KUBO, Kimio MONMA, Toshihiro NAGAYAMA, Akio OGATA and Hisashi KAMIMURA |
| genetically modified soybean, chromosome analysis, rat, chinese hamster |
| Ann.Rep.Tokyo Metr.Inst.P.H., 54,357-362,2003 |