13 Studies on Daily Intake of Food Additives - Sodium Saccharin, Aspartame, Glycyrrhizic Acid and Free Amino Acids -

YUKIKO YAMAJIMA, CHIGUSA KOBAYASHI, IKUKO OHNO, YUKINARI TATEISHI, MIYUKI KAWANO, YUKA KAWAI, MITSUO NAKAZATO, KAZUO YASUDA, MOTOHIRO NISHIJIMA

food additives, daily intake, market basket method, sodium saccharin, aspartame, glycyrrhizic acid, amino acids

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,69-74,2000

14 An Analytical Method for Stevia Sweeteners Stevioside in Food by High-Performance Liquid Chromatography

HIDEKI OZAWA, MASAKO HIROKADO, YASUHIRO SHIMAMURA, KAZUO NAKAJIMA, KEISUKE KIMURA, KAZUO YASUDA

A simple and efficient method for detecting and determining stevioside and rebaudioside A for sweeteners in beverages and food is described. Stevioside and rebaudioside A were extracted with water from a sample by dialyzing for two days. The extract was cleaned up with a Bond Elut C18 cartridge. Clean-up was performed by washing the cartridge with 20% acetonitrile, and the eluate by 80% acetonitrile was collected and analyzed by high performance liquid chromatography (HPLC). Recoveries of stevioside and rebaudioside A in samples spiked at 10 µg/g were 74.3 - 96.8% and 69.0 - 96.6%, respectively. Relative standard deviations of samples (CV value) were 0.1 -3.9%. The proposed method was applied to analysis of 18 comercially available samples with indication of stevia. Stevioside or rebaudioside A was detected in all these samples except FURIKAKE (Japanese rice seasoning), at levels of 2.2 - 179.0 µg/g and 3.1 - 101.7 µg/g, respectively.

natural sweetener, stevioside, rebaudioside A, solid-phase extraction, dialysis, HPLC

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,75-79,2000

15 Determination of p-Hydroxybenzoic Acid Esters in Foods

MIYUKI KAWANO, MITSUO NAKAZATO, CHIGUSA KOBAYASHI, YUKIKO YAMAJIMA, IKUKO OHNO, KAZUO YASUDA

The concentrations of six kinds of p-hydroxybenzoic acid esters (PHBA-Es), namely, PHBA-methyl ester, PHBA-ethyl ester, PHBA-propyl ester, PHBA-isopropyl ester, PHBA-butyl ester and PHBA-isobutyl ester, were determined in various foods by high performance liquid chromatography. PHBA-Es were extracted from samples using a mixture of acetonitrile-isopropanol-ethanol (2:1:1). The crude extract was stored in a freezer set up at -20°C for 1hr, and separated into an organic layer and the residue. The organic layer was concentrated to 1 - 2 ml, then the concentrated solution was diluted ten times with water. The solution was cleaned up on a Sep-Pak Vac C18 cartridge. The cartridge was washed with water and a mixture of methanol - water (l:9). The PHBA-Es were eluted from the cartridge with the methanol - water (8:2) mixture. The six of PHBA-Es were separated on a Cosmosil 5C18-AR II column with a mobile phase of methanol-5 mmol/L citrate buffer (6:4), and detected at 254 nm. Recoveries of the PHBA-Es from various kinds of foods were 90.8 - 106%. The detection limits of the PHBA-Es were 10 µg/g in the samples. The proposed method was useful on ana1ysis of PHBA-Es in foods containing oils and fats.

p-hydroxybenzoic acid esters, preservative, HPLC

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,80-84,2000

16 Analysis of Natural Colours in Commercial Foods by High-Performance Liquid Chromatography

KAZUO NAKAJIMA, MASAKO HIROKADO, YASUHIRO SHIMAMURA, HIDEKI OZAWA, KEISUKE KIMURA, KAZUO YASADA

natural colours, HPLC, anthocyanins colour, lac colour, cochineal colour, gardenia yellow colour, carthamus yellow colour, turmeric oleoresin colour, monascus colour

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,85-90,2000

17 Detection of Coal Tar Dyes in Foods by TLC Using Aluminium Sheet

YOKO AOYAGI, TSUTOMU OGIWARA, EIKO AMAKAWA, SUKEJI SUZUKI

Thin layer chromatography (TLC) using an aluminium sheet for analysis of coal tar dyes in foods was studied. The chromatographic behaviors of the 12 permitted coal tar dyes and 8 non-permitted dyes using an aluminium sheet were the same as that using a glass plate in normal- and reversed-phase TLC. Dyes permitted in commercial foods were developed with good reproducibility in reversed-phase TLC using an aluminium sheet and a glass plate. Normal-phased TLC using an aluminium sheet and glass plate were influenced by admixturing in foods and Rf values were reduced. However, normal-phased TLC was useful for a screening test of 12 permitted coal tar dyes from commercial foods. Chromatograms on aluminium sheet and glass plate did not disappear durinng seven months under sheild light. TLC with an aluminium sheet could be used for analysis of coal tar dyes in foods correspounding to GLP.

aluminium sheet, glass plate, reversed-phase thin layer chromatography, normal-phase thin layer chromatography, food coal tar dyes, non-permitted dyes

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,91-95,2000

18 Determination of Inorganic Phosphates Used for Treatment of Vegetables by Spectrophotometry

KAYO HAGINO, NOBUO TAGUCHI, MITSUO OISHI, YOKO YAMADA, SUKEJI SUZUKI

inorganic phosphate, vegetable, bleach, spectrophotometry, quantitative analysis

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,96-99 ,2000

19 Analytical Procedure for Bromide in Agricultural Products and Foodstuffs by High-Performance Liquid Chromatography

YUKI SADAMASU, AKIHIRO IBE, SETSUKO TABATA, HIROYUKI MIYAKAWA, AKIKO YASUI, KAZUO YASUDA

A simple and rapid method using HPLC was developed in order to determine bromide residues in agricultural products and foodstuffs. A 10 g sample with 4%NaOH in ethanol solution was ashed at 500°C. The ashed residue was dissolved in water and cleaned up by a Bond Elut SAX and a Mega Bond Elut PRS. Bromide in the eluent was determined by HPLC-UV using an anion exchange resin column, Ion Pac AS12A. Recoveries of bromide ion spiked at 2 ppm and regulation limits of respective agricultural products were more than 80.0%, and the detection limit was 0.5 ppm in agricultural products. The method was applied to 87 samples of l2 kinds of agricultural products and foodstuffs, so that considerable bromide was detected from corns, kiwifruits, strawberries and chinese chestnuts as compared with that from other agricultural products. Particularly, 43.2 ppm bromide was detected in chinese chestnut. Morever, some of the foodstuffs were found to have considerable bromide but this finding was probably caused by other ingredients such as salt. None of the samples exceeded the regulation limits in Japan.

bromine, bromide, agricultural products, HPLC, anion exchange resin column

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,100-104,2000

20 Survey of Pesticide Residues in Vegetables and Fruits - Apr. 1999 - Mar. 2000 -

MAKI KOBAYASHI, TOSHIHIRO NAGAYAMA, ICHIRO TAKANO, MASAKO ITO, YASUHIRO TAMURA,YUKINARI TATEISHI, NAOKO KIMURA, KYOKO KITAYAMA, KAZUO YASUDA

Pesticide residues in 64 kinds of vegetables and fruits and 17 kinds of organically cultivated crops or the like were investigated. In several kinds of crops, residues of 6 kinds of organophosphorus insecticides, 4 kinds of carbamate insecticides, 2 kinds of organochlorine insecticides, 4 kinds of organochlorine fungicides, 1 kind of organonitrogen insecticide were detected. Concentrations of organophosphorus insecticides (Chlorpyrifos, CYAP, EPN, Prothiofos, Diazinon and MEP), organochlorine insecticides (Dicofol and Dieldrin), organochlorine fungicides (Captan, Iprodione, Procymidone and TPN), carbamate insecticides (BPMC, Methomyl, NAC and Thiodicarb), organonitrogen insecticide (Tebufenpyrad) were between 0.01 and 0.18 ppm in 11 kinds of crops, Tr. (below 0.01 ppm) and 0.42 ppm in 9 kinds of crops, 0.01 and 0.23 ppm in 11 kinds of crops and 0.06 ppm in 1 kind of crop, respectively. There were no samples exceeding the maximam standards for withholding registration or the tolerance for pesticide residues. Residues of organophosphorus insecticides (DDVP), organochlorine insecticides (Dicofol), fungicide (Captan, Procymidone and TPN) and carbamate insecticide (Methomyl) in 5 kinds of organically cultivated crops or the like were detected. Their concentrations were between 0.01 and 0.36 ppm.

pesticide residues, vegetables, fruits, organophosphorus pesticides, organochlorine pesticides, carbamate pesticides, insecticides, fungicides, organic cultivation

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,105-110,2000

21 Survey of Pesticide Residues in Imported Crops (Organochlorine, N-Methyl Carbamate and the Other Pesticides) - Apr. 1999 - Mar. 2000 -

MASAO ITO, TOSHIHIRO NAGAYAMA, ICHIRO TAKANO, YASUHIRO TAMURA, YUKINARI TATEISHI, NAOKO KIMURA, KYOKO KITAYAMA,KAZUO YASUDA

Pesicide residues in 238 imported crops were investigated from April 1999 to March 2000. Residues of organochlorine pesticides, 5 kinds of insecticides (Heptachlor epoxide, Dieldrin, total- DDT, Dicofol and Endosulfan) and 3 kinds of fungicides (Captan, Iprodione and TPN) were detected between Trace and 1.6 ppm. As for carbamate pesticides, 2 kinds of insecticides (NAC and Methomyl) and one kind of herbicide (CIPC) were detected between 0.01 and 0.45 ppm. In the others, 3 kinds of fungicides (Imazalil, OPP and TBZ) and one kind of herbicide (2,4-D) were detected at levels between a trace and 6.3 ppm. Residues of these pesticides were at level lower than the tolerance for residues in Japan and the tolerance for pesticide residues in origin and CODEX maximum residues limits for pesticides. As for the kind of detected pesticides in imported crops, there was scarcely any difference among 5 regions. A difference based on the kind of imported crops was recognized.

pesticide residues, imported crops, organochlorine pesticides, carbamate pesticides, insecticides, fungicides, herbicides, postharvest application

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,111-117,2000

22 Survey of Pesticide Residues in Imported Crops (Organophosphorus and Organonitrogen Pesticides), Apr. 1999 - Mar. 2000

ICHIRO TAKANO, TOSHIHIRO NAGAYAMA, MAKI KOBAYASHI, MASAKO ITO, YASUHIRO TAMURA, YUKINARI TATEISHI, NAOKO KIMURA, KYOKO KITAYAMA, KAZUO YASUDA

Organophosphorus and organonitrogen pesticide residues in 238 imported crops were investigated. In fourteen kinds of crops, residues of 9 kinds of organophosphorus insecticides and 3 kinds of organonitrogen fungicides were detected. Concentrations of organophosphorus insecticides (Chlorpyrifos, Ethion, Methidathion, etc.) and organonitrogen fungicides (Bitertanol, Triadimefon and Triadimenol) were between a trace and 0.82 ppm in 20 crops and a trace and 0.03 ppm in 3 crops, respectively. Six kinds of pesticides detected in 9 kinds of crops are regulated by the tolerance for pesticide residues by the Food Sanitation Law. 0.04 ppm of Para-thion-methyl, which has been designated as a specified poisonous substance in Japan, was detected in pimento imported from New Zealand. Residues of these pesticides were at levels lower than the tolerance for pesticide residues, the standard for withholding registration of pesticides in Japan, the CODEX maximum residue limits for pesticides or the tolerance in each country.

pesticide residues, imported crops, organophosphorus pesticides, organonitrogen pesticides, postharvest application

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,118-123,2000

23 Determination of Fenpropathrin in Agricultual Products by Using the Examination Method Shown in Japanese Food Sanitation Law

YASUHIRO TAMURA, TOSHIHIRO NAGAYAMA, ITIRO TAKANO, MAKI KOBAYASHI, MASAKO ITO, YUKINARI TATEISHI, NAOKO KIMURA, KYOKO KITAYAMA, KAZUO YASUDA

Fenpropathrin in agricultural products was determined using the examination method shown in the Japanese Food Sanitation Law for nine pesticides including BHC, DDT, Aldrin and etc. The sample (grain, beans, vegetable or fruit) was homogenized with acetone folowed by extraction with n-hexane, and the extract was evaporated. In the case of grain or beans, fat or oil was removed from the extract by n-hexane acetonitrile partitioning. Then the extract was purified by Florisil column chromatography and analyzed by GC-ECD. Recoveries of fenpropathrin spiked in all crops (tomato, lemon, soybean, tea and other samples) studied at the 0.01 or 0.1 ppm level were ranged from 74.8 to 99.0%. The limit of detection was 0.005 ppm.

fenpropathrin, pesticide, agricultural producuts, gas chromatography (ECD) , examination method shown in Japanese Food Sanitation Low

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,124-127,2000

24 Survey of Pesticide Residues in Domestic Vegetables - Apr. 1996 - Mar. 2000 -

CHIEKO TAKADA, NORIO OHASHI, HIROSHI SATO, EIKO AMAKAWA, MICHIKO MIYAKODA, TSUTOMU OGIWARA, YOKO AOYAGI, SUKEJI SUZUKI

Pesticide residues in 42 kinds of crops and 9 kinds of frozen foods were investigated. Residues of 6 kinds of organochlorine insecticides, 5 kinds of organochlorine fungicides, 7 kinds of organophosphorus insecticides, 1 kind of organophosphorus fungicide, 1 kind of organonitrogen fungicide and organonitrogen herbicide were detected. Concentrations of organochlorine insecticides (Benzoepin, Heptachlor epoxide, Dicofol, DDT, DDE and Dieldrin), organochlorine fungicides (TPN, Procymidone, Iprodione, Vinclozolin and Captan), organophosphorus insecticides (EPN, Prothiofos, DDVP, Phosalone, MEP, Dimethoate and Ethion), organophosphorus fungicide (Tolclofos-methyl), organonitrogen fungicide (Dichlofluanid) and organonitrogen herbicide (Pendimethalin) were trace - 0.60 ppm in 7 kinds of crops, trace - 1.57 ppm in 11 kinds of crops, trace - 0.25 ppm in 10 kinds of crops, 0.02 - 0.51 ppm in 2 kinds of crops, 0.02 - 0.l5 ppm in 2 kinds of crops and 0.01 ppm in 1 kind of crop respectively. There were no carbamate or other pesticides detected.

pesticide residues, vegetables, organophosphorus pesticides, organochlorine pesticides, carbamate pesticides, organonitrogen pesticides, insecticides, fungicides, harbicides

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,128-134,2000

25 Determination of DDTs and Dicofol Residues in Vegetables by GC/MS and HPLC

HIROSHI SATOH, NORIO OHASHI, CHIEKO TAKADA, EIKO AMAKAWA, MICHIKO MIYAKODA, TSUTOMU OGIWARA, YOKO AOYAGI, SUKEJI SUZUKI

The analytical method to determine o, p'-DDT, p,p'-DDT (DDTs) by GC/MS and dicofol by HPLC in vegetables was studied. DDTs and dicofol were extracted with acetone and internal standard (DDT -d8) was added to acetone extract. The acetone extract was evaporated under reduced pressure. Residual aqueous solution was mixed with 6% NaCl solution, then shaked with n-hexane. The organic layer was evaporated and the residue was cleaned up with Envi-carb/LC-NH2 cartridge. In young soybeans, Extrelut 3 was used for removal of lipids. Stable isotope of DDTs was used as an internal standard by GC/MS-SIM. This proposed method showed good recoveries. Using HPLC, dicofol was separated from DDTs and their metabolites using a Zorbax C8 column with methanol-water-acetic acid (80:20:0.2) as the mobile phase, then detected at 230 nm. Dicofol, which was difficult to determine accurately because of pyrolysis in GC/MS analysis, could be determined quantitatively and showed good recoveries by HPLC. In frozen young soybeans and kidney beans, DDTs and dicofol, which may be endocrine disrupting chemicals, were detected.

pesticide residues, vegetables. endocrine disrupting chemicals, DDT, dicofol, stable isotope standard, GC/MS, HPLC, photodiode array detector

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,135-139,2000

26 Survey of Organochlorine Pesticide Residues in Imported Eel Products

TAKEO SASAMOTO, HIDEKI HASHIMOTO, TSUNEO HASHIMOTO, TOMOYUKI MIYAZAKI

Organochlorine pesticide residues in 54 imported eel products were investigated. These pesticides were extracted with acetone-petroleum ether (1:2). The extract was purified by column chromatography on Florisil® and defatted by gel permeation chromatography (GPC). All compounds were determined using gas chromatograph with an electron capture detector (GC-ECD) or a mass spectrometer (GC/MS). Nine pesticides and their isomers were detected. p,p'-DDE was the major component of DDT compounds in eel products. Residue concentrations of organochlorine pesticides in eel products processed in China were generally higher than that of these from other countries. The highest residue concentration was 5.34 ppm γ-HCH (Lindane) in a sample processed in China. The Residue of γ-HCH was over the maximum residue limits (MRL) of meat fat recommended by the FAO/WHO Codex Alimentarius Commission (1986).

organochlorine pesicides, residues, eel products

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,140-143,2000

27 Organochlorine Pesticide Residues in Short-necked Clam from Tokyo Bay - April 1994 - March 2000 -

HIDEKI HASHIMOTO, TSUNEO HASHIMOTO, TAKEO SASAMOTO, KUNIHIRO KAMATA, TOMOYUKI MIYAZAKI

organochlorine pesticides, short-necked clam, Tokyo Bay, endocrine disruptors, GC/MS, selected ion monitoring (SIM)

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,144-149,2000

28 The Comparison of Cadmium, Copper and Arsenic Concentrations between Unpolished and Polished Rice

HARUKICHI ONOZUKA, KIYOHIDE EBATO, TAKASHI AMEMIYA, KAZUKO MIZUISHI, YASUSHI ONO, TAKASHI FUJII, KAZUO ONISHI

rice, unpolished rice, polished rice, pollution, cadmium, copper, arsenic, permissible value, distribution of concentration, agricultural soil pollution

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,150-154,2000

29 Hygienic Studies on Imported Fish Sause (II) - Contents of 9 Elements -

MITSUO NAKAZATO, YUKINARI TATEISHI, CHIGUSA KOBAYASHI, YUKIKO YAMAJIMA, IKUKO OHNO, YUKA KAWAI, KAZUO YASUDA

fish sause, seasoning, arsenic, cadmium, lead, copper, zinc, iron, manganese, magnesium, calcium

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,155-159,2000

30 Determination of Bisphenol A in Canned Tea Drinks by HPLC with an Electrochemical Detector

HIROYUKI MIYAKAWA, AKIHIRO IBE, SETSUKO TABATA, HIDEKI OZAWA, YUKI SADAMASU, KAZUO YASUDA

A method for determining bisphenol A (4,4'-isopropylidene diphenol) in canned tea drinks using HPLC with an electrochemical detector was developed. Bisphenol A was extracted from samples using diethyl ether, and the extract was cleaned up with a Sep-Pak NH2 cartridge. Thereafter, bisphenol A was determined by HPLC. The detection limit was 1.0 ng/g. Twenty-four brands of commercial canned tea drinks were analyzed using this method. Bisphenol A was detected from 9 brands, and ranged from 1.0 to 82.5 ng/g. As the inside coating material was distinguished by FT-IR, the detection frequency of bisphenol A was high when the inside coating of the can body was a kind of epoxy resin. On the other hand, bisphenol A was not detected when the inside coating of the can body was polyethylene terephthalate.

bisphenol A, electrochemical detector, canned tea drinks, epoxy resin, poly (vinyl chloride) resin, polyethylene terephthalate (PET)

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,160-165,2000

31 Outbreaks of Food Poisoning of Chemical and Naturally Occurring Toxicants in Tokyo (XVII), 1999

HIROFUMI USHIYAMA, KIMIKO KAN, TETSUYA SHINDO, KAZUO YASUDA

chemical food poisoning, histamine, Lagenaria siceraria, cucurbitacin, detergent, surface active agent

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,166-169,2000

32 Radioactive Contamination in Imported Foods (IX) - Apr. 1999 - Mar. 2000 -

KIMIKO KAN, HIROFUMI USHIYAMA, TETSUYA SHINDO, KAZUO YASUDA

Chernobyl reactor accident, radioactive contamination, imported foods, survey, cesium, mushroom, black tea, NaI(Tl) scintillation detector

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,170-174,2000

33 Identification of Human and Animal Hair Detected as Foreign Substance in Food

KEISUKE KIMURA, MASAKO HIROKADO, KAZUO YASUDA, MOTOHIRO NISHIJIMA

hair, scanning electron microscope, microphotograph, mouse, cuticle scale, medulla of hair shaft, foreign substance

The external appearance, cuticle scale and cross section of human, mouse and another animal hair were observed using a microscope, micro CCD camera and electron microscope. These characteristics were summarized and a table was developed. Human and animal hair detected as foreign substance in bean paste, bread and syumai were tested and identified using this table. It was found that these hairs were mouse and human hair.

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,175-179,2000

34 Vitamin Content of Health Food (Drinks)

MASAO IGUCHI, NARUE SAKAMAKI, YUKI SAITO, KIMIO MONMA, NORIHISA KIKUTANI, FUSAO USHIO, HISATSUGU ICHIKAWA, TOMOKO MASUDA, MARIKO YOSHIZAWA

Fifteen samples of health food (drinks) sold in Japan were analysed for water-soluble and fat-soluble vitamin content in order to estimate vitamin intake. The amounts of vitamins per serving size were B1 0.1 - 9.2 mg, B2 0.3 - 7.4 mg, niacin 6 - 36 mg, nicotinamide 14 - 38 mg, B6 0.5 - 20 mg, B12 1.6 - 14 µg, pantothenic acid 3 - 10 mg , folate 148 - 160 µg, C 33 - 1,300 mg, A 212 - 750 IU, beta-carotene 1.3 mg, D 10 - 33 IU and E 0.7 - 44 mg. Compared with the tolerable upper intake level of Recommended Dietary Allowances for Japanese, the amounts of vitamin content in most samples were low except for one sample in which the niacin level (36 mg/100 ml) exceeded the upper intake level (33 mg).

health food, drink, vitamin, water-soluble vitamin, fat-soluble vitamin

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,180-183,2000

35 Studies on Fat Contents and Fatty Acids Composition of Instant Noodles

NARUE SAKAMAKI, MASAO IGUCHI, NORIHISA KIKUTANI, HISATSUGU ICHIKAWA

Fifty-one kinds of instant noodles (fried type：41, not fried type：8 and not dried, fresh type：2) were analysed for fat content and fatty acid composition. Total fat was measured by the method of ether extraction after acid treatment. Fatty acid composition were determined by gas-liquid chromatography. Average contents of fat per serving were 17.4 g in fried type, 6.8 g in not fried type and 7.4 g in not dried, fresh type. Main fatty acid of fat in fried type noodles were palmitic (C16：0) and oleic (C18：1) acids and the mean values of them were 37.4%, 40.7%, respectively. As most of the fatty acid composition of these fats was similar to that of palm oil, it was noted that mainly palm oil may have been used to fry these noodles.

instant noodles, deep fried noodles, fat content, saturated fatty acid, fatty acid composition

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,184-187,2000

36 Analysis of Thiabendazole and Imazalil in Natural Food Additives

KENJI IIDA, KEIKO HIRATA, YOKO UEMATSU, KUMI SUZUKI, TERUO KAN, KAZUO SAITO

Thiabendazole (TBZ) and Imazalil (IMZ) in natural food additives, such as color, antioxident etc, were determined by HPLC. Using condition of 3 methods extraction depending on the form of natural food additives, TBZ and IMZ were extracted with ethyl acetate from the sample. After test solutions were prepared by the liquid-liquid distribution of the extract, and were analyzed by HPLC. IMZ and TBZ were detected by ultraviolet detector at 220 nm, and fluorescence detector at excitation 305 nm, emission 350 nm, respectively. Recoveries and coefficients of variation of TBZ and IMZ from natural food additives spiked at 0.1 µg/g of TBZ and 5.0 µg/g of IMZ were in the range of 70.8-84.5%, 62.4 - 75.3%, and 3.9 -9.8%, 3.5 -13.4%, respectively. TBZ and IMZ in 69 samples of commercial food additive products were below the detection limits. (TBZ < 0.05 µg/g, IMZ < 1.0 µg/g)

natural food additives, thiabendazole, imazalil, HPLC

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,188-192,2000

37 Contents of Heavy Metals and Arsenic in Natural Food Additives

TETSUKO YASUNO, TERUHIKO HAGIWARA, KAZUO SAITO

heavy metal, lead, cadmium, chromium, mercury, arsenic, natural food additive

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,193-196,2000

38 Hygienic Studies on Pectin Digests as Natural Preservative

KOICHI ITO, HIROSHI HUZITA, KEIKO HIRATA, YOKO UEMATSU, KUMI SUZUKI, KENZI IIDA, KAZUO SAITO, MASAKO HIROKADO, KAZUO YASUDA

pectin digests, mutagenicity, ames test, salmonella typhimurium TA100, oligogalacturonic acid, HPLC, natural preservative

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,197-202,2000

39 Effects of Alkaline Cleanser on Migration of Bisphenol A from Nursing Bottles

KEIICHI FUNAYAMA, YUJI WATANABE, REIKO KANEKO, KAZUO SAITO

polycarbonate, bisphenol A, nursing bottle, alkaline cleanser, migration test

Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,203-207,2000

Title List

Reports IV Papers on Environmental Hygiene