1 Detection of Small Round-Structured Viruses from Gastroenteritis Patients
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MAMI NAGASHIMA, YASUKO YOSHIDA, YUKIKO TABEI, MICHIYA HASEGAWA, YUKINAO HAYASHI, KOHJI MORI, YUKIKO SASAKI, YAYOI NOGUCHI, ICHIRO HIRATA, HIROMASA SEKINE, YATARO KOKUBO, SATOSHI MOROZUMI |
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small round-structured virus (SRSV), acute gastroenteritis |
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Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,3-7,2000 |
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2 Outbreaks of Gastroenteritis Associated with Norwalk-like Viruses Occurred in Tokyo (1997.11-2000.3)
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YUKINAO HAYASHI, KOHJI MORI, YAYOI NOGUCHI, YUKIKO SASAKI, ATSUKO NAKAMURA, MAMI NAGASHIMA, YASUKO YOSHIDA, ICHIRO HIRATA, HIROMASA SEKINE, SATOSHI MOROZUMI |
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In May 1997, the Ministry of Health and Welfare regarded Norwalk-like viruses (NLVs)as the causative
agents of a gastroenteritis outbreak. According to the change in the Food Sanitation Law which added NLVs
as causative agents, we started to diagnose NLVs infection by two steps in the Tokyo Metropolitan Area.
RT-PCR for screening and dot-blot hybridyzation for confirmatory test of NLVs infection. Between November
1997 and March 2000, 5124 samples from 413 outbreaks of gastroenteritis were tested as viral causative
agents. Of these, 1465 samples (28.6%) from 247 outbreaks (59.8%) were positive for NLVs.
Recently, the number of gastroenteritis outbreaks occurring in nursing schools, kindergardens,
elementary schools, and nursing homes for elderly persons have increased. Contaminated shelfish, most
important vehicle of NLVs-related outbreak of gastroenteritis , were not served in these cases. In most of
these cases , there were signs of person-to-person spread of infection rather than food-poisoning. The
importance of virological testing during outbreaks of gastroenteritis will increase in the future to determine if
the outbreaks were caused by food poisoning.
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Norwalk-like viruses, gastroenteritis, outbreak, genotype |
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Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,8-13,2000 |
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3 The Transition of Influenza Antibody in the Elderly Who Inoculated the Vaccine (1997-1999)
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TAKAYUKI SHINKAI, KIYOSHI YAMAZAKI, KENJI SADAMASU, ATSUKO NAKAMURA, ICHIRO HIRATA, HIROMASA SEKINE, YATARO KOKUBO，SATOSHI MOROZUMI, TAKASHI INAMATSU |
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Influenza virus, Influenza vaccine, elderly, Hemagglutination inhibition test, Hemagglutination inhibition antibody titer |
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Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,14-19,2000 |
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4 Unstableness of ELISA Method for Chlamydia trachomatis Antibody
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IKU ISE |
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Enzyme Linked Immunosorbent Assay (ELISA), inactivation, Chlamydia trachomatis (CT) |
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Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,20-23,2000 |
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5 Results of Serological Diagnosis for Syphilis on Healthy Persons in Tama, Tokyo (1978-1999)
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ISAO YOSHIDA, KEIKO SUZUKI, ICHIRO HIRATA, KAZUE OGATA, REI KATOH, TADAHIKO ITO, SUMIO YAMADA |
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Between 1978 and 1999, serological screening for syphilis was performed on serum specimens presented from
45,614 healthy persons in Tama, Tokyo.
In screening tests with modified VDRL slide test and Ogata's method or Rapid Plasma Reagin Card (RPR)
test, 1,017 cases (2.23%) showed positive reaction.In addition,by confirmation with Treponema Pallidum
Hemagglutination (TPHA) test and FTA-ABS method, 639 cases (1.40%) appeared to be an inapparent infection. Of
these, 77.2% (493 cases) were persons of 60 years of age and more. Furthermore, 148 (0.9%) out of 16,365 negative
serum samples screened between 1988 and 1999 showed positive reactions with TPHA and/or FTA-ABS,
indicating a previous infection.
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syphilis, serologic test for syphilis, rapid plasma reagin test, treponema pallidum hemagglutination test |
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Ann.Rep.Tokyo Metr.Res.Lab.P.H., 51,24-26,2000 |